|
KMID : 0545119970070060435
|
|
Journal of Microbiology and Biotechnology
1997 Volume.7 No. 6 p.435 ~ p.437
|
|
|
Ca^2+ is Required to Make Functional Malate Synthase in Corynebacterium glutamicum
|
|
Kim, Hyung Joon
Kim, Jae Ho/Lee, Heung Shick
|
|
|
Abstract
|
|
|
|
The role of Ca^2+ in making functional malate synthase in Corynebacterium glutamicum was investigated using the cloned DNA coding for the enzyme. Introduction of cloned aceB into C. glutamicum overexpressed malate synthase as judged by SDS-PAGE. However, the increase in enzyme activity of the expressed malate synthase did not match the level of overexpression observed in SDS-PAGE. Addition of Ca^2+ to the growth medium specifically increased the activity. The malate synthase could be stained with ruthenium red in a Ca^2+-specific manner. This agrees with the previous observation which reported a potential Ca^2+-binding domain in the N-terminal region of the protein.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|